A Quantitative 1H NMR Method for Screening Cannabinoids in CBD Oils

Toxicologically relevant levels of the psychoactive ?9-tetrahydocannabinol (?9-THC) as well high non-psychoactive cannabinoids potentially occur in CBD (cannabidiol) oils. For consumer protection fast-growing oil market, facile and rapid quantitative methods to determine cannabinoid content are crucial. However, current standard method, i.e., liquid chromatography combined with tandem mass spectrometry (HPLC-MS/MS), requires a time-consuming multistep sample preparation. In this study, nuclear magnetic resonance spectroscopy (qNMR) method for screening oils was developed. Contrary HPLC-MS/MS qNMR features simple preparation, only diluting deuterochloroform. Pulse length-based concentration determination (PULCON) enables direct quantification using an external standard. The signal intensities were enhanced during NMR spectra acquisition by means multiple suppression triglycerides which major component matrix. validation confirmed linearity CBD, cannabinol (CBN), ?9-THC ?8-THC hemp seed sufficient recoveries precision screening. Comparing results data 46 commercial verified accuracy but higher limits detection. developed paves way increasing throughput complementary before HPLC-MS/MS.